Adenovirus-relaxin gene therapy for keloids: Implication for reversing pathological fibrosis

W. J. Lee, Y. O. Kim, I. K. Choi, D. K. Rah, C. O. Yun

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Summary Background Keloids or hypertrophic scars are pathological proliferations of the dermal skin layer resulting from excessive collagen deposition. Because the hormone relaxin (RLX) inhibits collagen synthesis and expression in stimulated fibroblasts, an adenovirus expressing RLX (dE1-RGD/lacZ/RLX) was generated. Objectives To investigate the effect of RLX-expressing adenovirus on expression of various extracellular matrix (ECM) components in primary keloid spheroids. Methods The expression levels of type I and III collagen, fibronectin and elastin were investigated by immunohistochemistry in primary keloid spheroids transduced with the RLX-expressing adenovirus. Results Immunohistochemical analysis showed that expression of major ECM components (e.g. type I and III collagen, elastin and fibronectin) was markedly reduced in primary keloid spheroids transduced with dE1-RGD/lacZ/RLX. Conclusions These results suggest that the antifibrotic effect of RLX-expressing adenovirus may have therapeutic effects on keloids by reversing pathological fibrosis and preventing keloid recurrence after surgical excision.

Original languageEnglish
Pages (from-to)673-677
Number of pages5
JournalBritish Journal of Dermatology
Volume165
Issue number3
DOIs
StatePublished - Sep 2011

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