A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system

Minseok S. Kim; Seyong Kwon; Eun Sook Lee; Je Kyun Park

A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system

Minseok S. Kim
, Seyong Kwon
, Eun Sook Lee
, Je Kyun Park

Department of New Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microflui-dics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages	696-698
Number of pages	3
State	Published - 2010
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010

Publication series

Name	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume	1

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/Territory	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Immunohistochemistry
Multiplexed diagnosis
Proteomic profiling

Cite this

Kim, M. S., Kwon, S., Lee, E. S., & Park, J. K. (2010). A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 696-698). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).

Kim, Minseok S. ; Kwon, Seyong ; Lee, Eun Sook et al. / A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 696-698 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

@inproceedings{9021fc2d38144a75aa71fae9d19274cf,

title = "A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system",

abstract = "This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microflui-dics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.",

keywords = "Breast cancer, Immunohistochemistry, Multiplexed diagnosis, Proteomic profiling",

author = "Kim, \{Minseok S.\} and Seyong Kwon and Lee, \{Eun Sook\} and Park, \{Je Kyun\}",

year = "2010",

language = "English",

isbn = "9781618390622",

series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

pages = "696--698",

booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",

}

Kim, MS, Kwon, S, Lee, ES & Park, JK 2010, A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 1, pp. 696-698, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 3/10/10.

A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system. / Kim, Minseok S.; Kwon, Seyong; Lee, Eun Sook et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 696-698 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system

AU - Kim, Minseok S.

AU - Kwon, Seyong

AU - Lee, Eun Sook

AU - Park, Je Kyun

PY - 2010

Y1 - 2010

N2 - This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microflui-dics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.

AB - This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microflui-dics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.

KW - Breast cancer

KW - Immunohistochemistry

KW - Multiplexed diagnosis

KW - Proteomic profiling

UR - https://www.scopus.com/pages/publications/84884297678

M3 - Conference contribution

AN - SCOPUS:84884297678

SN - 9781618390622

T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

SP - 696

EP - 698

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Kim MS, Kwon S, Lee ES, Park JK. A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 696-698. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system

Abstract

Publication series

Conference

UN SDGs

Keywords

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