@inproceedings{9021fc2d38144a75aa71fae9d19274cf,
title = "A novel method to investigate proteomic profiling of cancers using a microfluidic immunohistochemistry system",
abstract = "This paper presents a massively multiplexed and quantitative immunohistochemistry (IHC) platform using microflui-dics technology. The device was fabricated with two-step multiplayer soft lithography and a microvalve was integrated to simultaneously flow 10 biomarkers into individual reaction channel. The microfluidic massive IHC system enabled 20 IHC assays on a biological specimen at the same time, saving 16-fold time consumption. Moreover, the expressions of the biomarkers were quantitatively compared. The immunohistochemical staining from the microfluidic system showed accurate localization and comparable quality with that of conventional IHC method. Proteomic profiling was examined for 4 breast cancer cell lines, AU-565, HCC70, MCF-7 and SK-BR-3. The microfluidic massive IHC system is expected to be useful for accurate histopathological diagnosis using numerous specific biomarkers at a time.",
keywords = "Breast cancer, Immunohistochemistry, Multiplexed diagnosis, Proteomic profiling",
author = "Kim, {Minseok S.} and Seyong Kwon and Lee, {Eun Sook} and Park, {Je Kyun}",
year = "2010",
language = "English",
isbn = "9781618390622",
series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
pages = "696--698",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",
}
