A nonisotopic assay method for hepatitis C virus NS5B polymerase

Chanhee Park; Younghoon Kee; Jungchan Park; Heejoon Myung

doi:10.1016/S0166-0934(01)00443-8

A nonisotopic assay method for hepatitis C virus NS5B polymerase

Chanhee Park, Younghoon Kee, Jungchan Park, Heejoon Myung

Department of New Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The RNA-dependent RNA polymerase of hepatitis C virus (HCV) is responsible for replication of genomic RNA. A novel nonisotopic assay method is described for detecting its enzymatic activity. The 5′ end of the in vitro-transcribed template RNA was attached covalently to the surface of a Covalink module using carbodiimide condensation. The RNA strand containing the 3′ untranslated region (3′ UTR) of HCV at its 3′ end was free in the solution. A purified NS5B polymerase and NTPs along with biotin-labeled UTP were added to this module and the polymerization activity could be detected colorimetrically with streptavidin-conjugated alkaline phosphatase.

Original language	English
Pages (from-to)	211-214
Number of pages	4
Journal	Journal of Virological Methods
Volume	101
Issue number	1-2
DOIs	https://doi.org/10.1016/S0166-0934(01)00443-8
State	Published - 2002

Keywords

Covalink module
Hepatitis C virus
NS5B polymerase
Nonisotopic assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S0166-0934(01)00443-8

Cite this

@article{11d4ce501a9948f0808021bcbd3644b3,

title = "A nonisotopic assay method for hepatitis C virus NS5B polymerase",

abstract = "The RNA-dependent RNA polymerase of hepatitis C virus (HCV) is responsible for replication of genomic RNA. A novel nonisotopic assay method is described for detecting its enzymatic activity. The 5′ end of the in vitro-transcribed template RNA was attached covalently to the surface of a Covalink module using carbodiimide condensation. The RNA strand containing the 3′ untranslated region (3′ UTR) of HCV at its 3′ end was free in the solution. A purified NS5B polymerase and NTPs along with biotin-labeled UTP were added to this module and the polymerization activity could be detected colorimetrically with streptavidin-conjugated alkaline phosphatase.",

keywords = "Covalink module, Hepatitis C virus, NS5B polymerase, Nonisotopic assay",

author = "Chanhee Park and Younghoon Kee and Jungchan Park and Heejoon Myung",

year = "2002",

doi = "10.1016/S0166-0934(01)00443-8",

language = "English",

volume = "101",

pages = "211--214",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - A nonisotopic assay method for hepatitis C virus NS5B polymerase

AU - Park, Chanhee

AU - Kee, Younghoon

AU - Park, Jungchan

AU - Myung, Heejoon

PY - 2002

Y1 - 2002

N2 - The RNA-dependent RNA polymerase of hepatitis C virus (HCV) is responsible for replication of genomic RNA. A novel nonisotopic assay method is described for detecting its enzymatic activity. The 5′ end of the in vitro-transcribed template RNA was attached covalently to the surface of a Covalink module using carbodiimide condensation. The RNA strand containing the 3′ untranslated region (3′ UTR) of HCV at its 3′ end was free in the solution. A purified NS5B polymerase and NTPs along with biotin-labeled UTP were added to this module and the polymerization activity could be detected colorimetrically with streptavidin-conjugated alkaline phosphatase.

AB - The RNA-dependent RNA polymerase of hepatitis C virus (HCV) is responsible for replication of genomic RNA. A novel nonisotopic assay method is described for detecting its enzymatic activity. The 5′ end of the in vitro-transcribed template RNA was attached covalently to the surface of a Covalink module using carbodiimide condensation. The RNA strand containing the 3′ untranslated region (3′ UTR) of HCV at its 3′ end was free in the solution. A purified NS5B polymerase and NTPs along with biotin-labeled UTP were added to this module and the polymerization activity could be detected colorimetrically with streptavidin-conjugated alkaline phosphatase.

KW - Covalink module

KW - Hepatitis C virus

KW - NS5B polymerase

KW - Nonisotopic assay

UR - http://www.scopus.com/inward/record.url?scp=0036175506&partnerID=8YFLogxK

U2 - 10.1016/S0166-0934(01)00443-8

DO - 10.1016/S0166-0934(01)00443-8

M3 - Article

C2 - 11849700

AN - SCOPUS:0036175506

SN - 0166-0934

VL - 101

SP - 211

EP - 214

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

A nonisotopic assay method for hepatitis C virus NS5B polymerase

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this