A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment

Jae Youn Hwang; Sebastian Wachsmann-Hogiu; V. Krishnan Ramanujan; Julia Ljubimova; Zeev Gross; Harry B. Gray; Lali K. Medina-Kauwe; Daniel L. Farkas

doi:10.1007/s11307-011-0517-z

A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment

Jae Youn Hwang
, Sebastian Wachsmann-Hogiu
, V. Krishnan Ramanujan
, Julia Ljubimova
, Zeev Gross
, Harry B. Gray
, Lali K. Medina-Kauwe
, Daniel L. Farkas

Department of Electrical Engineering and Computer Science

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

Original language	English
Pages (from-to)	431-442
Number of pages	12
Journal	Molecular Imaging and Biology
Volume	14
Issue number	4
DOIs	https://doi.org/10.1007/s11307-011-0517-z
State	Published - Aug 2012

Bibliographical note

Funding Information:
Acknowledgements. We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson & Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO-07119), and NSF (BESOO 79483) to DLF.

Keywords

Chemotherapy
Corroles
Fluorescence lifetime
In vivo
Multimode
Nanoconstruct
Preclinical
Spectral analysis
Wide-field two-photon excitation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11307-011-0517-z

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Hwang, J. Y., Wachsmann-Hogiu, S., Ramanujan, V. K., Ljubimova, J., Gross, Z., Gray, H. B., Medina-Kauwe, L. K., & Farkas, D. L. (2012). A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment. Molecular Imaging and Biology, 14(4), 431-442. https://doi.org/10.1007/s11307-011-0517-z

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title = "A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment",

abstract = "Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.",

keywords = "Chemotherapy, Corroles, Fluorescence lifetime, In vivo, Multimode, Nanoconstruct, Preclinical, Spectral analysis, Wide-field two-photon excitation",

author = "Hwang, \{Jae Youn\} and Sebastian Wachsmann-Hogiu and Ramanujan, \{V. Krishnan\} and Julia Ljubimova and Zeev Gross and Gray, \{Harry B.\} and Medina-Kauwe, \{Lali K.\} and Farkas, \{Daniel L.\}",

note = "Funding Information: Acknowledgements. We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson \& Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO-07119), and NSF (BESOO 79483) to DLF.",

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T1 - A multimode optical imaging system for preclinical applications in Vivo

T2 - Technology development, multiscale imaging, and chemotherapy assessment

AU - Hwang, Jae Youn

AU - Wachsmann-Hogiu, Sebastian

AU - Ramanujan, V. Krishnan

AU - Ljubimova, Julia

AU - Gross, Zeev

AU - Gray, Harry B.

AU - Medina-Kauwe, Lali K.

AU - Farkas, Daniel L.

N1 - Funding Information: Acknowledgements. We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson & Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO-07119), and NSF (BESOO 79483) to DLF.

PY - 2012/8

Y1 - 2012/8

N2 - Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

AB - Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

KW - Chemotherapy

KW - Corroles

KW - Fluorescence lifetime

KW - In vivo

KW - Multimode

KW - Nanoconstruct

KW - Preclinical

KW - Spectral analysis

KW - Wide-field two-photon excitation

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DO - 10.1007/s11307-011-0517-z

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AN - SCOPUS:84865448782

SN - 1536-1632

VL - 14

SP - 431

EP - 442

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 4

ER -

A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment

Abstract

Bibliographical note

Keywords

UN SDGs

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