TY - GEN
T1 - A microfluidic multiplexed immunohistochemistry platform for quantitative pathological diagnosis
AU - Kim, Minseok S.
AU - Lee, Eunsook
AU - Kim, Chul Hwan
AU - Bae, Chae Yoon
AU - Kong, Sun Young
AU - Park, Je Kyun
PY - 2009
Y1 - 2009
N2 - A novel microfluidic multiplexed immunohistochemistry (MMIHC) platform was proposed for quantitative pathological diagnosis. Multilayer soft lithography and two-step lithography technologies were used to fabricate MMIHC devices. To overcome the subjective decision of immunohistochemistry (IHC) staining results, image analysis was conducted and quantified with staining intensity and stained area values. By using reversible sealing method between the device and a cellblock sample, not only perfect fluid control for various solutions was exhibited without any leakage, bubble formation and cross-contamination, but also intact sample condition in reaction areas and stable sample preservation were guaranteed. Biomarker examination for estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), progesterone receptor (PR) and Ki-67 was realized for SK-BR-3 breast cancer cell line at one time. In our experiment, it was revealed that the platform enhanced at least 200-fold reaction efficiency and 24-fold reduction of incubation time. These achievements expect not only savings of time, cost and tissue samples, expanded test for prognostic biomarker candidates and early personalized therapy, but also integration of genomic and proteomic approaches into search for targets in oncology.
AB - A novel microfluidic multiplexed immunohistochemistry (MMIHC) platform was proposed for quantitative pathological diagnosis. Multilayer soft lithography and two-step lithography technologies were used to fabricate MMIHC devices. To overcome the subjective decision of immunohistochemistry (IHC) staining results, image analysis was conducted and quantified with staining intensity and stained area values. By using reversible sealing method between the device and a cellblock sample, not only perfect fluid control for various solutions was exhibited without any leakage, bubble formation and cross-contamination, but also intact sample condition in reaction areas and stable sample preservation were guaranteed. Biomarker examination for estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), progesterone receptor (PR) and Ki-67 was realized for SK-BR-3 breast cancer cell line at one time. In our experiment, it was revealed that the platform enhanced at least 200-fold reaction efficiency and 24-fold reduction of incubation time. These achievements expect not only savings of time, cost and tissue samples, expanded test for prognostic biomarker candidates and early personalized therapy, but also integration of genomic and proteomic approaches into search for targets in oncology.
KW - Biomarker
KW - Immunohistochemistry
KW - Multilayer soft lithography
KW - Multiplexing
KW - Personalized medicine
UR - http://www.scopus.com/inward/record.url?scp=79951817754&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:79951817754
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1973
EP - 1975
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -