A droplet-based microfluidic system for high- Throughput screening of photosensitisers against microbial organisms

Soougwon Cho, Dong Ku Kang, Steven Sim, Florian Geier, Jin Young Kim, Soo Ik Chang, Joshua Edel, Robert Wootton, Andrew De Mello

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Herein, we present a modular droplet-basecl microfluidic approach for performing liigh throughput cytotoxicity screening of photo sensitizers against microbial organisms. Multiple novel fluidic operation modalities such as large-scale chamber based light irradiation, reinjection and low voltage driven electrocoalescence are introduced. Also, photosensitiser drug cytotoxicity on E.coli cells are evaluated in microfluidic device using fluorescent viability assay indicator and compared with conventional colony forming unit counting cytotoxicity assay.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages332-334
Number of pages3
ISBN (Print)9780979806452
StatePublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Droplet
  • Drug screening
  • Lab on a chip
  • Microfluidic
  • PDT
  • Photo dynamic

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