TY - JOUR
T1 - A Droplet-Based High-Throughput SERS Platform on a Droplet-Guiding-Track-Engraved Superhydrophobic Substrate
AU - Shin, Sera
AU - Lee, Jaehong
AU - Lee, Sanggeun
AU - Kim, Hyunchul
AU - Seo, Jungmok
AU - Kim, Dayeong
AU - Hong, Juree
AU - Lee, Soonil
AU - Lee, Taeyoon
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/2/17
Y1 - 2017/2/17
N2 - A novel droplet-based surface-enhanced Raman scattering (SERS) sensor for high-throughput real-time SERS monitoring is presented. The developed sensors are based on a droplet-guiding-track-engraved superhydrophobic substrate covered with hierarchical SERS-active Ag dendrites. The droplet-guiding track with a droplet stopper is designed to manipulate the movement of a droplet on the superhydrophobic substrate. The superhydrophobic Ag dendritic substrates are fabricated through a galvanic displacement reaction and subsequent self-assembled monolayer coating. The optimal galvanic reaction time to fabricate a SERS-active Ag dendritic substrate for effective SERS detection is determined, with the optimized substrate exhibiting an enhancement factor of 6.3 × 105. The height of the droplet stopper is optimized to control droplet motion, including moving and stopping. Based on the manipulation of individual droplets, the optimized droplet-based real-time SERS sensor shows high resistance to surface contaminants, and droplets containing rhodamine 6G, Nile blue A, and malachite green are successively controlled and detected without spectral interference. This noble droplet-based SERS sensor reduces sample preparation time to a few seconds and increased detection rate to 0.5 µL s−1 through the simple operation mechanism of the sensor. Accordingly, our sensor enables high-throughput real-time molecular detection of various target analytes for real-time chemical and biological monitoring.
AB - A novel droplet-based surface-enhanced Raman scattering (SERS) sensor for high-throughput real-time SERS monitoring is presented. The developed sensors are based on a droplet-guiding-track-engraved superhydrophobic substrate covered with hierarchical SERS-active Ag dendrites. The droplet-guiding track with a droplet stopper is designed to manipulate the movement of a droplet on the superhydrophobic substrate. The superhydrophobic Ag dendritic substrates are fabricated through a galvanic displacement reaction and subsequent self-assembled monolayer coating. The optimal galvanic reaction time to fabricate a SERS-active Ag dendritic substrate for effective SERS detection is determined, with the optimized substrate exhibiting an enhancement factor of 6.3 × 105. The height of the droplet stopper is optimized to control droplet motion, including moving and stopping. Based on the manipulation of individual droplets, the optimized droplet-based real-time SERS sensor shows high resistance to surface contaminants, and droplets containing rhodamine 6G, Nile blue A, and malachite green are successively controlled and detected without spectral interference. This noble droplet-based SERS sensor reduces sample preparation time to a few seconds and increased detection rate to 0.5 µL s−1 through the simple operation mechanism of the sensor. Accordingly, our sensor enables high-throughput real-time molecular detection of various target analytes for real-time chemical and biological monitoring.
KW - droplet-guiding system
KW - galvanic displacement reaction
KW - open-channel microfluidic
KW - sensors
KW - superhydrophobicity
KW - surface-enhanced Raman scattering
UR - http://www.scopus.com/inward/record.url?scp=85006377173&partnerID=8YFLogxK
U2 - 10.1002/smll.201602865
DO - 10.1002/smll.201602865
M3 - Article
AN - SCOPUS:85006377173
SN - 1613-6810
VL - 13
JO - Small
JF - Small
IS - 7
M1 - 1602865
ER -