96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network

Chaewon Jin; Jinyoung Kim; Hongsoo Choi

doi:10.1109/MEMS46641.2020.9056388

96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network

Chaewon Jin
, Jinyoung Kim
, Hongsoo Choi

Daegu Gyeongbuk Institute of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

In this study, we have established 5 different micro-organs (Cortex-Hippocampus-Heat-Liver-Tumor) network in vitro in the 96-well format-based microfluidic platform by physically separating but fluidically interconnecting spherical microtissues (MTs) via the microfluidic channel. An effect of the anti-cancer drug, 5-fluorouaracil (5-FU), on tumor MT and also other organotypic MTs was investigated with their interaction through microfluidic network in vitro for 3-day cultivation While no significant suppression was observed when tumor MTs were solely cultured in 5-FU, growth of tumor MTs was suppressed in the in vitro 5 micro-organ network without critical impact on the other 4 MTs. It addresses feasibility of our microfluidic platform for more complex and comprehensive pharmacokinetic study in an in vivo-like in vitro physiological environment.

Original language	English
Title of host publication	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1002-1005
Number of pages	4
ISBN (Electronic)	9781728135809
DOIs	https://doi.org/10.1109/MEMS46641.2020.9056388
State	Published - Jan 2020
Event	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada Duration: 18 Jan 2020 → 22 Jan 2020

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2020-January
ISSN (Print)	1084-6999

Conference

Conference	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Country/Territory	Canada
City	Vancouver
Period	18/01/20 → 22/01/20

Bibliographical note

Publisher Copyright:
© 2020 IEEE.

Keywords

3D cell culture
Co-culture
In-vitro micro-organ network
Microfluidics
Microtissue

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/MEMS46641.2020.9056388

Cite this

Jin, C., Kim, J., & Choi, H. (2020). 96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (pp. 1002-1005). Article 9056388 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056388

Jin, Chaewon ; Kim, Jinyoung ; Choi, Hongsoo. / 96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network. 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. pp. 1002-1005 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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title = "96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network",

abstract = "In this study, we have established 5 different micro-organs (Cortex-Hippocampus-Heat-Liver-Tumor) network in vitro in the 96-well format-based microfluidic platform by physically separating but fluidically interconnecting spherical microtissues (MTs) via the microfluidic channel. An effect of the anti-cancer drug, 5-fluorouaracil (5-FU), on tumor MT and also other organotypic MTs was investigated with their interaction through microfluidic network in vitro for 3-day cultivation While no significant suppression was observed when tumor MTs were solely cultured in 5-FU, growth of tumor MTs was suppressed in the in vitro 5 micro-organ network without critical impact on the other 4 MTs. It addresses feasibility of our microfluidic platform for more complex and comprehensive pharmacokinetic study in an in vivo-like in vitro physiological environment.",

keywords = "3D cell culture, Co-culture, In-vitro micro-organ network, Microfluidics, Microtissue",

author = "Chaewon Jin and Jinyoung Kim and Hongsoo Choi",

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year = "2020",

month = jan,

doi = "10.1109/MEMS46641.2020.9056388",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

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Jin, C, Kim, J & Choi, H 2020, 96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network. in 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020., 9056388, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2020-January, Institute of Electrical and Electronics Engineers Inc., pp. 1002-1005, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020, Vancouver, Canada, 18/01/20. https://doi.org/10.1109/MEMS46641.2020.9056388

96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network. / Jin, Chaewon; Kim, Jinyoung ; Choi, Hongsoo.
33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. p. 1002-1005 9056388 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - In this study, we have established 5 different micro-organs (Cortex-Hippocampus-Heat-Liver-Tumor) network in vitro in the 96-well format-based microfluidic platform by physically separating but fluidically interconnecting spherical microtissues (MTs) via the microfluidic channel. An effect of the anti-cancer drug, 5-fluorouaracil (5-FU), on tumor MT and also other organotypic MTs was investigated with their interaction through microfluidic network in vitro for 3-day cultivation While no significant suppression was observed when tumor MTs were solely cultured in 5-FU, growth of tumor MTs was suppressed in the in vitro 5 micro-organ network without critical impact on the other 4 MTs. It addresses feasibility of our microfluidic platform for more complex and comprehensive pharmacokinetic study in an in vivo-like in vitro physiological environment.

AB - In this study, we have established 5 different micro-organs (Cortex-Hippocampus-Heat-Liver-Tumor) network in vitro in the 96-well format-based microfluidic platform by physically separating but fluidically interconnecting spherical microtissues (MTs) via the microfluidic channel. An effect of the anti-cancer drug, 5-fluorouaracil (5-FU), on tumor MT and also other organotypic MTs was investigated with their interaction through microfluidic network in vitro for 3-day cultivation While no significant suppression was observed when tumor MTs were solely cultured in 5-FU, growth of tumor MTs was suppressed in the in vitro 5 micro-organ network without critical impact on the other 4 MTs. It addresses feasibility of our microfluidic platform for more complex and comprehensive pharmacokinetic study in an in vivo-like in vitro physiological environment.

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ER -

Jin C, Kim J , Choi H. 96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc. 2020. p. 1002-1005. 9056388. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS46641.2020.9056388

96-Well Format-Based Microfluidic Paltform for an in-Vitro Multiple Micro-Organ Network

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

Access to Document

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