3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation

Dong Ku Kang; Xiuqing Gong; Soongwon Cho; Jin Young Kim; Joshua B. Edel; Soo Ik Chang; Jaebum Choo; Andrew J. Demello

doi:10.1021/acs.analchem.5b02402

3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation

Dong Ku Kang, Xiuqing Gong, Soongwon Cho, Jin Young Kim, Joshua B. Edel, Soo Ik Chang, Jaebum Choo, Andrew J. Demello

Division of Biotechnology

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.

Original language	English
Pages (from-to)	10770-10778
Number of pages	9
Journal	Analytical Chemistry
Volume	87
Issue number	21
DOIs	https://doi.org/10.1021/acs.analchem.5b02402
State	Published - 3 Nov 2015

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Publisher Copyright:
© 2015 American Chemical Society.

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abstract = "Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating microdroplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.",

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AU - Kang, Dong Ku

AU - Gong, Xiuqing

AU - Cho, Soongwon

AU - Kim, Jin Young

AU - Edel, Joshua B.

AU - Chang, Soo Ik

AU - Choo, Jaebum

AU - Demello, Andrew J.

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3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation

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